Assessment Of Resistance To Colicinogenic Synthetic Phage Antimicrobial System

The unifying goal of this proposal is to lessen the burden of antimicrobial resistance (AMR) using a holistic framework. My research goal is to develop a synthetic-phage-based antimicrobial that is more specific and less suspectable to resistance than traditional broad-spectrum antibiotics. My learning module goal is to create a learning resource promoting AMR stewardship by providing the foundation knowledge required to appreciate the severity of the problem and demonstrate how cutting-edge biotechnology can be used to mitigate to solve it.My research objectives are the following:Engineer a synthetic T7 phage that co-expresses colicin-E1 and colicin-M (T7cea,cma)Determine the impact of additional hurdles on mutation rateCompare the resistance genes associated with the triple-, double-, and single-hurdle antimicrobials.My education objectivesare the following:Establish an AMR case study learning module for secondary education with the intention of increasing scientific literacy of AMR and demonstrating how biotechnology can be used to mitigate it.