Breeding of Aflatoxin Genetic Resistance in Maize

The objective of this project is to create and characterize new hybrid and synthetic maize varieties displaying high and stable resistance to Aflatoxin (A.) flavus and aflatoxin accumulation using field inoculation and ELISA assay, as well as other traits important to Central and Western African growing environments, including but not limited to resistance for Maize Lethal Necrosis (MLN) disease. Germplasm and genes identified previously and during the course of this project will be used in practical breeding programs to develop resistant varieties for developing world farmers within three years of the termination of this project. Inbred lines will be characterized for MLN resistance, and hybrids and Open pollinated varieties (OPVs) characterized for yield and aflatoxin and A. flavus resistance in growing conditions typical of African farmers; other traits will be screened as necessary, including local diseases. Specific and general combining ability will be tested, and information on performance of the lines in hybrid combinations in different environments will be made publically available to maize breeders and small seed companies. Seeds of up to 15 resistant inbred lines will be sent to The International Maize and Wheat Improvement Center (CIMMYT) for further distribution to interested parties.