The goal of this proposal is topinpoint the cluster homolog of immunoglobulin-like receptors (CHIR) localizing with signaling molecules (SHP-1 & SHP-2) in the intestineby leveraging single cell-RNA sequencing (scRNA-seq) and multiplex spatial profiling in a coccidiosis disease model.Objectives:1: Defining the intestinal cell populations using single-cell RNA-sequencing of the chicken in coccidiosis infection.This will be the first study to utilize single-cell sequencing technology to distinguish immune cell populations from the intestine of two distinct lines of B-haplotype chickens.2: Determining the localization and expressions of CHIR-B2, SHP-1, and SHP-2 in the chicken intestine in coccidiosis infection using multiplex spatial profiling.Understanding the specific immune cell types that express CHIR-B2, SHP-1, and SHP-2 will enable differentially expressed genes to be correlated to the immune response in coccidiosis. Information from this assay will be integrated with the single-cell RNA sequencing data to confirm cell types expressing these genes in their spatial relationship.
CHARACTERIZING THE SPATIAL EXPRESSION OF THE �CLUSTERED HOMOLOG OF IMMUNOGLOBULIN-LIKE RECEPTORS� (CHIR) IN THE CHICKEN INTESTINE AFTER COCCIDIOSIS VACCINE CHALLENGE
Objective
Investigators
Drechsler, Y.
Institution
WESTERN UNIVERSITY OF HEALTH SCIENCES
Start date
2023
End date
2025
Funding Source
Project number
WUCVMDrechsler2cocci
Accession number
1029833
Commodities