Chemical Determination of Mycotoxins in Field Samples and Culture Materials

Objective

To measure the levels of aflatoxins (B1, B2, G1, and G2) and fumonisin B and C series in field samples, especially corn and fungi such as Aspergillus flavus, A. parasiticus and Fusarium spp. Results of these levels will be used to provide data to evaluate approaches to control of toxin levels in the corn crop.

More information

The Mycotoxin Laboratory, University of Minnesota, St. Paul, MN will help us in measuring, analyzing, identifying, and confirming the types and the levels of aflatoxins (B1, B2, G1, and G2) and the fumonisins B and C series in corn, fungal cultures, and other field samples. These methods, such as GC/MS, LC/MS, GC, and LC/FAB/MS, and HPLC are needed because immunoassays (ELISA and affinity columns) measure the total of aflatoxins or total of fumonisins, but not individual toxins. Also, immunoassays give too much
cross-reactivity and interference between different forms of the toxins. GC/MS, LC/MS, PNMR, and C13NMR are also important in determining if any novel toxins exist in the samples and all these instruments are available at the University of Minnesota.

Investigators

Abbas, Hamed

Institution

University of Minnesota

Start date

2000

End date

2005

Funding Source

Agricultural Research Service

Project number

6402-42000-002-01S

Accession number

403152