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National Agricultural Library
U.S. Department of Agriculture
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  4. Colonization Characteristics of Bovine Recto-Anal Junction Tissue by Escherichia Coli O157:H7

Colonization Characteristics of Bovine Recto-Anal Junction Tissue by Escherichia Coli O157:H7

Objective

<OL> <LI> Develop an in vitro model system for the binding of E. coli O157:H7 to bovine recto-anal junction tissue. <LI>Characterize the binding of E. coli O157:H7 to the bovine recto-anal junction. <LI>Characterize the shedding patterns of E. coli O157:H7 with different tir alleles in cattle colonized at the recto-anal junction.

More information

Approach: Colons will be obtained from commercial beef processing plants to determine the specific bind of E. coli O157:H7 to the RAJ and then determine the mechanism of binding, whether virulence factors are related to binding, and whether non-pathogenic bacteria inhibit binding. We will determine whether different tir alleles result in different colonization of the RAJ and affect persistence of shedding.

Investigators
Bono, James
Institution
USDA - Agricultural Research Service
Start date
2007
End date
2008
Funding Source
Agricultural Research Service
Project number
5438-32000-028-04T
Accession number
412001
Categories
Viruses and Prions
Escherichia coli