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The principal investigator developed the pilot studies for this proposal by transporting mass spectrometry combined with pattern recognition technology to the CFSAN: <ol> <li>The biology of learning sets is required to develop a test system.
<li>Simple membrane-fractionation techniques will enhance numbers of identified proteins.
<li>Sub-typing of Vibrio parahemolyticus is transportable to other systems.
<li>The method can sub-type pathogenic from non-pathogenic E. coli strains.
<li>A joint FDA/IAOAC symposium to discuss the role of mass spectrometry in the rapid speciation of bacteria (June 2001).</ol>
FY 2001 Plans:<br>
Complete development of collaborative studies among personnel and equipmentlocated at CFSAN, NCTR and the University of Arkansas at Fayetteville, AR: 1) Evaluation of transport of technology to simpler TOF instruments. 2) Evaluation of transport of technology to MALDI utilizing FT (Fourier Transform) enabling high-resolution detection in larger molecular weight ranges. It is anticipated that one two-week assignment each quarter to CFSAN and preliminary experiments at the University of Arkansas at Fayetteville will enable these experiments.