DECIPHERING THE CYTOSOLIC CONTRIBUTION TO SHIKIMATE/QUINATE DERIVED METABOLITES

Goal 1:Fully biochemically characterize the DHDSDH isoforms from Arabidopsis, tomato, and beetObjective 1.1:Determine the full sequence of all transcripts of beet BvDHDSDH2Objective 1.2:Biochemically characterize DHDSDH isoformsObjective 1.3:Determine subcellular localization of DHDSDH isoformsGoal 2: Determine the in planta metabolic role of cytosolic DHDSDH isoforms using reverse genetic strategiesObjective 2.1: Determing metabolic impact of SlDHDSDH2 loss of functionObjective 2.2: Determine contribution of SlDHDSDH2 to plant herbivory resistanceObjective 2.3: Assess gain of function in a type-b-negative species3) Assess the non-canonical beet DHDSDH contribution to betalain metabolismObjective 3.1:Analyze DHDSDH gene expression in beetObjective 3.2: Test loss of function of BvDHDSDH isoforms