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The goal of this project is to increase food safety by developing the next generation of detection and analysis methodology for bacterial toxins in food, using Staphylococcus aureus enterotoxin A (SEA) as a model. The proposal aims to develop two technologies: a cell culture based assay of SEA activity and biosensor methodology for immediate automated detection of SEA in food.
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The two different approaches will complement each other since they address two sides of the same problem. Biosensor detection allows rapid detection of the toxin in food, while the cell culture methodology supplies the information on biological activity of the toxin. These two methods are expected to overcome the limitations of current immunological and animal-based tests for toxins in food.</p>
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The cell culture based activity assay will be developed by exploiting the toxin's ability to stimulate division of lymphocytes. Biosensor technology represents a new approach to food safety analysis: real-time analysis. Biosensors can translate biological measurements into electronic signals enabling immediate analysis and automation. A novel methodology applying biosensor technology to food testing is proposed here. </p>
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The aim of this project is to develop new testing methodologies which will aid food production and food regulation, and may increase food safety and quality.</p>