DETECTION AND TYPING OF FOOD-BORNE PATHOGENS

Develop protein- and nucleic acid-based methods for detection, and characterization of food-borne pathogens. Specifically, develop antibody- and oligonucleotide-based detection microarrays for multiple pathogens; and improved real-time multiplex PCR assays. Develop rapid and effective means to separate and concentrate targeted pathogens from food matrices that can be coupled to very rapid detection methods such as real-time PCR. Develop molecular typing methods for pathogens of concern to associated food regulatory agencies. Examine environmental factors and microbiological culture conditions affecting genotypes or phenotypes that are important for virulence, isolation, or detection of foodborne pathogens.