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National Agricultural Library
U.S. Department of Agriculture
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  4. Detection of Botulinum Toxin in Animal Sera and Organs

Detection of Botulinum Toxin in Animal Sera and Organs

Objective

1. To develop reagents for the sensitive detection of BoNTs in ELISA or ALISSA assay formats. <P> 2. To optimize techniques for the recovery and detection of toxin in animal tissues and sera. <P> 3. To determine the pharmacokinetics of botulinum neurotoxins in oral and parenteral mouse models of intoxication.

More information

Approach:
Monoclonal antibodies directed against botulinum neurotoxins (BoNTs) serotypes B, E and F will be produced and used in sensitive in vitro assays to detect toxin in animal sera and tissues. Animals will be intoxicated and the half life and tissue distribution of the toxins determined.

Investigators
Cheng, Luisa Wai Wai
Institution
USDA - Agricultural Research Service
Start date
2009
End date
2013
Funding Source
Agricultural Research Service
Project number
5325-42000-048-04R
Accession number
418580
Categories
Natural Toxins
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