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The broad objective of the proposed research entails an integrated approach to the development of high performance capillary electrophoresis-laser induced fluorescence (HPCE-LIF) detection methods for the sensitive and selective determination of naturally occurring glycoside toxins in foods and feeds, namely glucosinolates.
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Toward this end the following objectives will be pursued:
<ol> <li>Develop selective derivatization schemes for the sensitive determination of total glucosinolates via their glucose residue by HPCE-LIF;
<li>Introduce highly sensitive profiling schemes for glucosinolates in plant tissues by HPCE-LIF via the enzymatic or chemical degradation products of glucosinolates; and,
<li>Implement novel electrolyte systems for rapid detection by HPCE-LIF of the various products derived from glucosinolates.</ol> </p>
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Objectives (1) and (2) involves the fragmentation or modification of the glucosinolate molecules by enzymatic and/or chemical processes, and their subsequent chemical derivatization with fluorescent tags for detection by HPCE-LIF. </p>
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Objective (3) will be accomplished by evaluating and introducing novel electrolyte systems of various composition in order to yield rapid and efficient separation schemes for the highly sensitive detection of glucosinolates at low levels even in a single plant seed or a small plant tissue. </p>
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These timely undertakings will certainly be beneficial for the sustainability of the United States agriculture.The various ultra sensitive detection schemes by HPCE-LIF have the potentials of increasing the accuracy of the analysis of food and feed samples, and advancing the use of the new analytical technology HPCE-LIF in monitoring processes of relevance to food safety.</p>