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Detection of Toxoplasma Oocysts in Municipal Water

Objective

To develop test for the detection of Toxoplasma gondii in environmental water supplies.

More information

Toxoplasma gondii oocysts will be obtained from feces of experimentally infected cats, purified by sucrose-gradients. Oocysts will be sporulated in 2% sulfuric acid as preservative and then stored at 4 C until used. Known numbers of oocysts will be mixed with water samples and then sensitivity of various methods (bioassay, PCR, monoclonal antibodies) will be tested.

Investigators
Dubey, Jitender
Institution
Uniformed Services University of the Health Sciences
Start date
1998
End date
2003
Project number
1265-32000-066-04R