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National Agricultural Library
U.S. Department of Agriculture
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  4. Detection of Viable Foodborne Pathogens by Bacteriophage/Bioluminescent Sensors

Detection of Viable Foodborne Pathogens by Bacteriophage/Bioluminescent Sensors

Objective

1. Genetically construct pathogen specific bacteriophage capable of stimulating and enhancing bioluminescence from bioluminescent bioreporter cell populations for the detection, monitoring, and quantitative sensing of microbial pathogenic species. 2. Apply bacteriophage/bioluminescent bioreporter systems with high throughput microtiter plate readers and portable field-based photomultiplier units. 3.Develop quantitative RT-PCR verification protocols for mRNA from the recombinant bacteriophage.

Investigators
Applegate, Bruce
Institution
Purdue University
Start date
2019
End date
2004
Funding Source
Nat'l. Inst. of Food and Agriculture
Project number
IND060046
Accession number
188147
Categories
Bacterial Pathogens
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