DEVELOPING CRISPR-BASED IN VITRO FUNCTIONAL GENOMIC SCREENING RESOURCES IN CHICKENS

The goal of this projectis to develop and validate user-friendly CRISPR-based in vitro screening resources for chickens, including cell lines, plasmids, protocols, data, and training, and share these resources with the research community. We will achieve this through the following three specific Aims:Aim1: Establish and optimize in vitro CRISPR-based transcription activation (CRISPRa) systems in different lineages of chicken cell linesAim2: Establish and optimize in vitro CRISPR-based transcription repression (CRISPRi) systems in different lineages of chicken cell linesAim3: Establish and optimize CRISPRa and CRISPRi high-throughput single-cell based CRISPR functional screening (scCRISPR-seq)