DEVELOPING IMPROVED LEAFY GREEN SALAD CROPS, FOR FIELD AND INDOOR GROWING SYSTEMS

This aim of this research project is to develop improved leafy green crops for California that are nutrient dense, including with enhanced anti-cancer properties, have a reduced environmental footprint, particularly with respect to water consumption and a longer shelf life, thus reducing waste and are safe with reduced tendency for microbial contamination. The specific objectives are:-To establish novel genomic and genetic resources in lettuce and watercress enabling investigation of a wide range of traits. In particular to sequence the genome of watercress, develop the first molecular genetic map. In lettuce to identify novel sequence variants for traits of interest.-Identify QTL for anti-oxidant potential, extended shelf-life, anti-cancer chemistry (watercress), water use efficiency (WUE), the good microbiome for safe food and shelf life.-Characterize genes underlying QTL, develop robust molecular markers for the traits of interest.-Develop proof-of-concept CRISPR-Cas approaches in lettuce to test traits of interest.-Work in vertical agricultural systems to determine the best phenotypes for indoor agriculture with optimized nutrition and WUE.