Development and Evaluation of a Portable Nanopore-based Sensing Device for Rapid Detection and Subtyping of Microbial Foodborne Pathogens

The overarching goal of this project is to develop and evaluate a promising nanopore sensing platform that has shown potential in clinical protein biomarkers for viruses and bacteria. The first objective involves developing a nanopore-based construct/device capable of sensitively detecting human noroviruses. The second objective involvesdeveloping a nanopore-based construct capable of sensitively detectingSalmonella enterica. The third and final objective involves evaluating the ability of this OmpG nanopore sensing system to subtype different strains of noroviruses and serotypes of Salmonella enterica, as well as confirming the specificity of the OmpG sensors against a panel of related and enteric viruses and bacteria.