An official website of the United States government.

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

National Agricultural Library
U.S. Department of Agriculture
  1. Home
  2. Research Tools
  3. Food Safety Research Projects Database
  4. DEVELOPMENT OF A CRISPR-CAS14 G-QUADRUPLEX (CRISPR-G4) BIOSENSOR FOR THE DETECTION OF NOROVIRUS IN LEAFY GREENS

DEVELOPMENT OF A CRISPR-CAS14 G-QUADRUPLEX (CRISPR-G4) BIOSENSOR FOR THE DETECTION OF NOROVIRUS IN LEAFY GREENS

Objective

The project aims to develop a CRISPR-Cas14-G4 system for the detection of NoV in leafy greens. The specific goals are listed below:Objective 1 - Develop a CRISPR-Cas14 system for the fluorescent detection of NoV RNA1.1 Design a CRISPR-Cas14 system1.2 CRISPR-Cas14-based detection of NoV RNAObjective 2 - Develop a CRISPR-Cas14-G4 sensor for NoV RNA detection2.1 Optimization of the CRISPR-G4 system2.2 Detection of NoV RNA with the CRISPR-G4 sensorObjective 33.1 Viral infection of leafy greens3.2 Detection of NoV in leafy greens

Investigators
Kasputis, T.
Institution
VIRGINIA POLYTECHNIC INSTITUTE
Start date
2024
End date
2026
Funding Source
Nat'l. Inst. of Food and Agriculture
Project number
VA-Kasputis
Accession number
1032517
https://portal.nifa.usda.gov/web/crisprojectpages/1032517.html