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National Agricultural Library
U.S. Department of Agriculture
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  4. DEVELOPMENT OF A GENETIC PERTURBATION PLATFORM FOR GRAM-POSITIVE BACTERIA

DEVELOPMENT OF A GENETIC PERTURBATION PLATFORM FOR GRAM-POSITIVE BACTERIA

Objective

Objective 1. Optimization of protein electroporation conditions with Leuconostoc citreum, a representative Gram-positive bacteria. In order to easily evaluate electroporation conditions, we will use fluorescently-labeled BSA as a target protein (FITC-BSA). Electroporation efficiency will be evaluated using flow cytometry and fluorescence microscopy. Conditions will be tested with a full factorial design and will include the number of cells, voltage, and resistance.

Investigators
Miller, Michael; Yong-su, Ji, .
Institution
University of Illinois
Start date
2020
End date
2021
Funding Source
Nat'l. Inst. of Food and Agriculture
Project number
ILLU-698-931
Accession number
1023511
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