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Development of an HPLC Method for Simultaneous Determination of Intense Sweeteners

Objective

The study aims to develop and validate a method for the simultaneous measurement of acesulfame K, saccharin, aspartame and neohesperidine dihydrochalcone (NHDC) in foodstuffs; and a separate method for the determination of cyclamate in foodstuffs.The simultaneous method incorporates dialysis extraction of aqueous dispersions of samples followed by determination of the analytes by ion pair reversed phase HPLC with UV detection. The method will be validated by collaborative trial.

More information

Intense sweeteners are being used to an increasing extent as replacements for sugar in foodstuffs. Following implementation of Directives 94/35/EC and 96/83/EC on the use of sweeteners in foodstuffs, a need was identified to provide suitable methodology for determining these substances in foodstuffs in order to allow monitoring of the intake of sweeteners from the diet to be carried out. As different sweeteners are often used in combination in the same food or drink, a method is needed for the simultaneous determination of the sweeteners most commonly used in foods.

<p>Find more about this project and other FSA food safety-related projects at the <a href="http://www.food.gov.uk/science/research/&quot; target="_blank">Food Standards Agency Research webpage</a>.

Institution
Central Science Laboratory
Start date
2000
End date
2003
Funding Source
Project number
A01012