<p>There are reliable methods for the determination of antioxidants in edible oils and fats, but these are not immediately applicable to other foods. Extraction of the antioxidant or mixture of antioxidants from some foods is not straightforward as the antioxidant may be encapsulated or bound within the food matrix. Furthermore, some methods of liberating antioxidants may actually lead to their destruction or loss. In this project, the antioxidants will be extracted from foods by several methods including steam distillation and the extracts analysed by an established technique such as IUPAC 2.642. The best method will be optimised for each food type and the final protocol validated by collaborative trial. In addition to the use of traditional wet chemistry techniques for the extraction of antioxidants from foods, the project aims to trial the novel technique of developing molecular imprinted polymers (MIPS) against some of the antioxidants under consideration.</p>
<p>Background: Antioxidants are permitted in a limited range of foods, and at well-defined levels as laid down by the Miscellaneous Food Additives Regulations 1995 (as amended). An antioxidant is a substance that is used to delay, retard or prevent the development in food of rancidity or other flavour deterioration due to oxidation. Antioxidants are added to foods to prevent deterioration of the food by atmospheric oxidation. This is particularly important in oils and fats, and in foods containing oils and fats, to prevent rancidity developing on storage. There is a need for accurate methods for the measurement of these antioxidants in foods both for regulatory enforcement and for monitoring of the intake of additives by the population. </p>