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Develop multi-analyte biosensors (multiple members of a drug or chemical family) that may be coupled directly on-line for the detection of residues in foods and feed. The methods developed should be of direct use to federal regulatory and/or action agencies.
<p>FY01 Program Increase $269,370. 1SY Added.
Development of adequate sampling protocols is a critical component of any detection scheme. Methods will be developed that result in separation of pathogens from an aggregate matrix or biofilm, and simultaneously enrich and concentrate multiple pathogenic species. Extraction and concentration is essential for a biosensor that does not rely on a preenrichment culture step. Pathogens will be prepared and concentrated using techniques such as sequential filtration, gravitation, immunotrapping, ligand-receptor interactions, and cell sorting. If preentrichment is necessary, methods will be developed to shorten the incubation times required. Methods such as integrated optical sensing, mass spectrometry, sensitive fluorescence, immuno-electrochemical detection, immunomagnetic electrochemiluminesce, microchip arrays, and combinatorial chemistry will be adapted to meet these goals. Reagents, such as monoclonal antibodies, receptors, and molecular imprints will be generated to facilitate separation and detection schemes.
<p>FY01 Program Increase $269,370. 1SY Added.