Development of Rapid Detection Methods Using Nanobiosensors for Salmonella and Vaccine Against Se and St for Poultry

Objective

To develop a nanobiosensor to detect Salmonella using surface-enhanced Raman scattering (SERS) technique: (1) determine SERS characteristics of Salmonella; (2) develop chemometric models to differentiate Salmonella from other bacteria; (3) classify different Salmonella strains; and (4) evaluate detection limits of nanobiosensor.

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Approach:
We will develop methodologies to test and validate the SERS techniques. A series of experiments will be conducted based on the following methods: 1) Design, fabricate SERS substrate to optimize for lower detection limits; 2) Salmonella sample preparation; 3) SERS measurement; 4) Test and validate classification models for different serotypes; 4) Test detectability from different background flora.

Investigators

Park, Bosoon

Institution

USDA - Agricultural Research Service

Start date

2010

End date

2015

Funding Source

Agricultural Research Service

Project number

6040-42000-041-03T

Accession number

420863