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National Agricultural Library
U.S. Department of Agriculture
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  4. DEVELOPMENT OF RAPID, FLEXIBLE, AND COST-EFFECTIVE MOLECULAR DIAGNOSTIC METHODS FOR COVID-19 DETECTION IN ANIMALS

DEVELOPMENT OF RAPID, FLEXIBLE, AND COST-EFFECTIVE MOLECULAR DIAGNOSTIC METHODS FOR COVID-19 DETECTION IN ANIMALS

Objective

This project aims to develop and validate molecular methods for detecting and sequencing SARS-CoV-2 in animal samples. We propose RT-LAMP and rhAmpSeq to detect and sequence SARS-CoV-2 variants, respectively, from farmed animal samples.The three specific aims are below.Aim 1: To develop an RT-LAMP assay for the SARS-CoV-2 detection in animal samples:Aim 2: To develop a rhPCR MinION-based rapid and cost-effective method for sequencing SARS-CoV-2 variants in animals:Aim 3: To validate RT-LAMP and rhPCR Minion Sequencing with RT-PCR and Illumina sequencing.

Investigators
Kuchipudi, S. V.
Institution
PENNSYLVANIA STATE UNIVERSITY
Start date
2023
End date
2026
Funding Source
Nat'l. Inst. of Food and Agriculture
Project number
PENW-2022-11225
Accession number
1030155
https://portal.nifa.usda.gov/web/crisprojectpages/1030155.html