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National Agricultural Library
U.S. Department of Agriculture
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  4. Development of Rapid Tests for the Detection of Mycobacterium Avium subsp. Paratuberculosis (MAP) and Their Use to Determine Survival in Food Matrices

Development of Rapid Tests for the Detection of Mycobacterium Avium subsp. Paratuberculosis (MAP) and Their Use to Determine Survival in Food Matrices

Objective

<OL> <LI> Optimisation of a PCR/DNA probe assay for the detection of MAP in faeces for clinical and sub-clinically infected animals.
<LI>Optimisation of sample preparation methods to enable the sensitive application of the PCR/DNA probe assay for the detection of MAP in faeces
<LI>Validation of the application of the assays for the identification of MAP in infected/suspect heerds.
<LI>Optimization of a NucliSens assay for the detection of MAP RNA in milk as a monitor for the presence of viable organisms in milk.
<LI>Determination of the heat resistance of MAP in milk.

More information

Progress: Monclonals have been raised but further work is required to find the most suitable for further development. Data has been obtained on heat resistance but as yet insufficient to complete a response surface model. A RT-PCR assay has been developed but further work is required to optimize the method. A real time assay for MAP has been developed but its value has still to be validated using naturally infected samples.

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Institution
Queen's University - Belfast
National University of Ireland, Galway (NUI Galway)
Start date
2001
End date
2004
Funding Source
safefood
Project number
00-RESR-060