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Development of Simple, Rapid, Immunoassays and DNA Based Confirmatory Tests for Marine Fish Speciation

Objective

The last three years have seen a steady decline in the quantity of fish landed by the UK fleet, however the 748 thousand tonnes of sea fish and shellfish landed in 2000 were still worth about 550 million. The demersal fish accounted for 49% of the catch in terms of quantity and 59% in value. Cod, haddock, whiting and plaice together accounted for more than half of the demersal species landed in the UK. However it is the quantity of cod and haddock landed that has seen the greatest decline. This shortage of supplies and correspondingly higher prices provides the potential for substitution of the traditionally popular varieties with cheaper, lower quality alternatives.
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In most cases it is relatively easy to identify whole fish and to distinguish some fish portions, however once processed further any distinguishing morphological features are no longer recognisable and it is impossible to confidently determine the species used. Therefore there is a need for the development and application of analytical methods to detect mislabelling and provide the consumer with accurate information about the nature and quality of seafood products offered for sale.

More information

This project aims to develop screening and confirmatory tests for the speciation of ten major marine fish. The tests will utilise the presence of particular proteins. Specific proteins will be identified, isolated from representative samples of fish flesh and monoclonal and polyclonal antibodies will be produced. Novel techniques will be used to manipulate the binding characteristics. Antibodies will be characterised by enzyme linked immunosorbent assay (ELISA), and the most appropriate combinations in terms of specificity and affinity identified. Suitable formats will be developed for the production of commercial screening assays. The assays will take the form of an inexpensive microtitration plate system.
<p>Find more about this project and other FSA food safety-related projects at the <a href="http://www.food.gov.uk/science/research/&quot; target="_blank">Food Standards Agency Research webpage</a>.

Institution
Institute of Food Research, UK
Start date
2002
End date
2004
Funding Source
Project number
Q01068