Development of Technologies for Detection and Mitigation of Undesirable Organisms Associated with Food

Approach:
1. Develop high quality monoclonal antibodies against toxin-producing bacteria. 2. Develop new assays for toxin-producing bacteria using immunological methods that can be used to solve practical problems facing the food industry and regulatory agencies. 3. Develop multiplex assays (antibody chips and immune-PCR) for simultaneous detection of multiple pathogens. 4. All results obtained will compare to conventional culture method and PCR results. 5. Using non-pathogenic E. coli K-12 strains as surrogates to observe the radiation sensitivity of the most common pathogens encountered in ground beef, milk, egg, fruits and vegetables. 6. Specific product loading pattern will be determined based on the results obtained from experiments. These results include: orientation of the product with respect to the conveyor flow and electron beam, unit count within the package, package dimensions and mass, the orientation of product within the package, and acceptance variations in these parameters. 7. The zone of minimum and maximum dose within a product load will be identified and the reproducibility of the process will be assessed. This information will be then used in selecting the dose monitoring locations for routine processing. 8. Evidence will be obtained to show that the finished product is accepted for its intended use after exposure to radiation. 9. The ability of the finished product to remain acceptable for intended use throughout its shelf life after exposure to the maximum radiation dose will be demonstrated. 10. Pathogens for biological validation will use E. coli O157:H7, Salmonella and Listeria monocytogenes. 11. To ensure the safety of food packaging materials exposed to irradiation will be selected to comply with FDA 21 Code of Federal Regulations (CFR) 179.