EFFECT OF CYTOPLASMIC POLYADENYLATION ELEMENT PROTEIN OVEREXPRESSION ON THE DEVELOPMENT OF BOVINE CLONED EMBRYOS

Our long-term goal is to improve cloning efficiency by overexpressing CPEB1 in bovine cloned embryos. The overall objectives of this proposal are to determine the role of CPEB1 in bovine embryogenesis and identify its effects on gene expression profiles in cloned embryos injected with CPEB1.The three objectives are:1. Determine CPEB1 expression levels at different developmental stages of IVF embryos derived from high and low quality of oocytes.2. Identify the effect of overexpression of CPEB1 on the development of IVF embryos derived from high and low quality of oocytes.3. Identify the effect of overexpression of CPEB1 on the development of cloned embryos and their gene expression profile.