THE EFFECT OF PROBIOTICS USE IN FOOD ANIMALS ON GUT AND FECAL MICROBIOME AND ANTIMICROBIAL RESISTANCE OF FOODBORNE PATHOGENS

The overall objective of this proposal is to study the effect of using the probiotics (live microbes) for food animals to enhance the microbiome (microbial diversity) in gut and fecal matter to force out the pathogenic microbes which are the potential source of microbial contamination.The aim of the current study: To investigate the changes in the microbiome composition after exposure to probiotics treatment, and AMR transfer dynamics. To achieve the study aims, several approaches will be used including the 16S rRNA gene sequencing to determine bacterial taxonomy of gut microbiota populations; shotgun metagenomics analyses to identify the diversity of genes present within the microbiota, such as AMR genes and those associated with HGT; and meta-transcriptomic analyses for profiling of community-wide gene expression (RNA-seq) to evaluate factors that impact to the development and spread of AMR.