<ol> <li> Determine the persistence of Salmonella in swine manure as affected by dietary
treatment. <li> Identify genes important for Salmonella colonization of the swine
gastrointestinal tract and persistence in manure.</ol>
Approach: <ol> <li> The effects of dietary treatment on the duration of Salmonella persistence in
stored swine manure will be determined and a correlation between Salmonella
persistence in manure and the native microbial community will be performed. <li> The
role of quorum sensing on Salmonella swine colonization, shedding, and persistence in
manure will be determined. <li> Genes required for Salmonella survival and
persistence in swine manure will be elucidated.