THE EFFECTS OF SEASONALITY AND CALLOSE DEPOSITION ON CARBON TRANSPORT IN CROP PLANTS

1. Conduct a two seasonal studiesof callose deposition after drought and delayed winter dormancy.a. Determine turgor loss points for the target species.b. Measure chlorophyll fluorescence, callose presence, callose location, phloem anatomy, leaf stomatal conductance, leaf photosynthetic rate on the target species once per season.c. record phenological stages for the greenhouse population and outdoor conspecifics seasonally: noting the timing of leaf bud break, floral bud break, open leaves, open flowers, yellow leaves, and leave abcission.c. In the second year, also measure phloem sap flow rate for target species during each seasonal timepoint.2. Analyze the data from each study3. Prepare manuscripts describing the outcome of each study