The overall research goal is to develop an enzyme-based processing strategy utilizing chlorogenic acid esterase (CE) to hydrolyze chlorogenic acid (CGA) and thereby prevent greening in sunflower seed-derived products. The specific objectives are to:(1) Use directed protein evolution strategies to engineer CE from Lactobacillus johnsonii for enhanced activity and stability.(2) Determine the enzymatic activity of engineered CE towards CGA and its ability to degrade CGA in sunflower seed-derived ingredients and prevent greening.(3) Scale-up enzyme production in a fermenter for future industrial application of engineered CE.
ELIMINATING UNDESIRED GREENING IN SUNFLOWER SEED DERIVED INGREDIENTS BY HYDROLYSIS OF CHLOROGENIC ACID USING AN ENGINEERED ESTERASE
Objective
Investigators
Were, L. M.; Owens, Ce, P..
Institution
Chapman University
Start date
2020
End date
2022
Funding Source
Project number
CALW-2019-06807
Accession number
1022168
Commodities