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Make publicly available a predictive computer program for mycotoxin levels in Midwest corn and adapt as necessary for a wider range of human food use corn varieties. <P>
Use molecular biological approaches to discover, introduce, and evaluate new insect resistance genes (producing bioactive proteins and secondary metabolites), alone and in combination. <P>
Use molecular biological approaches to discover, introduce, and evaluate plant-derived selectable markers for transgnic plant production.
Approach:
As part of the process of making the program publicly available, feedback on the present state of the program will be sought and utilized as appropriate. An economic module to assist in making control decisions will be developed and incorporated. The program will be tested for utility in food grade corn by comparing actual field collected data with predicted levels and correcting as necessary empirically. Genes identified in the prior project that are potentially useful in combination will be examined in model systems and further evaluated. New genes of potential use will be identified through functional targeting of cDNA, array-based technology. Molecular evolution of genes coding for resistance proteins will be utilized to further optimize efficacy against insects, while at the same time minimizing vertebrate effects. Gene product efficacy will be examined in model systems and in regenerated corn. Plant-derived genes involved in toxin resistance will be the ultimate focus of the investigation, although genes from other sources will initially be examined if appropriate plant-derived gene sequence information is not yet sufficient for cloning. Efficacy of target gene products as selectable markers and against insects, alone and in combination with insect-active genes, will be investigated.