Evaluation of Detection Methods for Shiga-Toxin Producing Escherichia coli and Salmonella from Cattle Fecal Samples, Retail Meat, and Fresh Produce

Shiga toxin producing Escherichia coli (STEC) and/or Salmonella are ranked as the most prevalent foodborne pathogens in the US. The objective of this cooperative research project is to compare different methods of detecting STEC and/or Salmonella to identify the most efficient and reliable methods in cattle fecal samples, meat, and fresh produce and apply the selected methods to real world samples. The experiments will produce information on the best enrichment procedure, most suitable selection media, and the sensitivity and selectivity of the methods to enhance surveillance efficiency of food borne pathogens to ensure food safety. New targets will be identified for rapid screening assays development.