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To challenge the feasibility of the system prototype by conducting side-by-side confirmatory analyses of shared samples with the U. of Kentucky cooperator using state-of-the-art lab-based techniques (advanced chromatography and mass spectrometry). For sample preparation, the QuEChERS method will be employed for a wide range of potential analytes and matrices. This method can be adapted for the analysis of identified marker molecules in target matrices to counter possible food security threats.
Approach:
University of Kentucky PI is using proprietary chemical-based methods to detect chemicals in a variety of food samples. ARS shall collaborate in a “red team – blue team” approach in which ARS will be the red team. The red team is tasked with challenging the blue team with possible scenarios that should be covered by the blue team’s monitoring system, and the blue team must address the challenge or adapt to it. After initial testing and method validation, a double-blind study will be conducted to assess the system performance and readiness for official testing.