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National Agricultural Library
U.S. Department of Agriculture
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  4. GENETIC ENGINEERING COTTON FOR ENHANCED RESISTANCE TO ASPERGILLUS FLAVUS

GENETIC ENGINEERING COTTON FOR ENHANCED RESISTANCE TO ASPERGILLUS FLAVUS

Objective

Identify cotton plastid promoters that demonstrate high expression levels in both green and non-green plant tissues for use in development of cotton plastid transformation vectors. Determine levels of expression of reporter genes in both green (leaf and outer boll) and non-green (cottonseed and root) cotton tissues under control of select, engineered plastid promoters. Generate cotton plastid transformation vectors that place antifungal genes and selectable marker genes under control of selected cotton plastid promoters and transform cotton. Test transformed tissues for expression of antifungal genes and selectable marker genes under both light and dark growth conditions. Perform in planta bioassays for antifungal activity in transplastomic cotton plants.

Investigators
Cary J W
Institution
University of Louisiana
Start date
2008
End date
2011
Funding Source
Agricultural Research Service
Project number
6435-42000-021-06S
Accession number
413466
Categories
Mycotoxins
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