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National Agricultural Library
U.S. Department of Agriculture
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  4. Genetic Modification of T7 Bacteriophage For The Rapid, Electrochemical and Broad Host Range Detection of E. Coli in Agricultural Water

Genetic Modification of T7 Bacteriophage For The Rapid, Electrochemical and Broad Host Range Detection of E. Coli in Agricultural Water

Objective

1) Successful genetic modification of the bacteriophage T7 as the delivery agent for an enzymatic reporter that can besubsequently expressed in infected E. coli.2) Characterization of reporter enzyme levels and impact of electrochemical signal amplification.3) Correlation of signal to E. coli levels and determination of the assays limit of detection.4) Successful detection by T7-assay of E. coli in agricultural water samples.

Investigators
Alcaine, S. D.
Institution
Cornell University
Start date
2016
End date
2017
Funding Source
Nat'l. Inst. of Food and Agriculture
Project number
NYC-143530
Accession number
1010308
Categories
Escherichia coli
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