How Salmonella Enteritidis changes virulence and resistance during mild processing: implications in shell egg pasteurization

1. Determining the changes in the transcription of genes encoding (a) general and specific stress regulators and (b) virulence, when Salmonella Enteritidis is grown in shell eggs and exposed to different heating rates and ozone treatments.2.Assessing the virulence expression in mouse model when infected with Salmonella Enteritidis grown in shell eggs and treated with heat, ozone and heat-ozone combination.3.Determining the heat resistance, measured as D-value, of Salmonella Enteritidis grown in shell eggs and treated with heat and ozone.