Identification of Genetic Markers for Resistance to Aspergillus flavus Growth and Aflatoxin Accumulation

The overall objective of this research is to identify genes encoding resistance associated proteins (RAPs), e.g., antifungal, stress responsive, or interfering with toxin signaling pathways, in developing and mature corn kernels through transcriptional profiling of Aspergillus flavus infected corn seed. We also will examine the effect of environmental changes on growth, development and aflatoxin production to better predict the durability of resistance to Aspergillus flavus and aflatoxin accumulation in response to climate change. The specific objectives are: a) Identify specific fungal genes involved in the plant-fungal interactions and aflatoxin production; b) Identify specific corn genes involved in the plant-fungal interactions; specifically, those involved in resistance to fungal invasion and aflatoxin production; c) Determine molecular bases of elevated CO2 concentrations and temperature on reproduction in Aspergillus flavus.