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The objectives of this agreemnet are to (1) determine the quantitative effects of various particle characteristics on the overall efficiency of detecting E. coli 0157:H7 in hamburger or coders by Threshold instrument according to developed procedures and (2) standardize the procedure for potential adoption by interested customers.
In general, magnetic particles of different physical properties (size, density) will be treated to contain approximately the same surface concentration of streptavidin. The particles are then used to 0157:H7 antibodies are then used to link to the streptavidin. The particles are then used to capture target pathogens spiked in hamburger or other foods. The capture will be measured by one of the biosensor techniques available at ERRC. The capture efficiency will be determined and correlated to the physical and chemical properties of the applied particles. Standard procedures with acceptable reproducibility for using the most efficient beads will then be developed for easy adoption by general laboratory personnel.