IMPROVED BIOLOGICALLY-BASED METHODS FOR MANAGEMENT OF NATIVE AND INVASIVE CROP INSECT PESTS

Objective 1: Develop genetically modified (GM) strains by transposon and CRISPR/Cas-mediated transgenesis for improved SIT in fruit fly pests, and new DNA delivery systems to apply this technology to a wider range of insect, including emerging pest species (e.g., caribfly, mexfly, medfly, spotted-winged drosophila). Objective 2: Develop strains of moths transinfected with Wolbachia that produce males with strong cytoplasmic incompatibility for use in the Incompatible Insect Technique (IIT) to reduce pest populations of fall armyworm and corn earworm. Objective 3: Develop CRISPR gene editing in pest moths (e.g., Indian meal moth, fall armyworm, gypsy moth) that target genes critical for acquired biopesticide resistance using both whole insects and cultured insect cells. Objective 4: Develop improved surveillance and detection methods for hidden and invasive pests (e.g., red palm and citrus root weevil, Asian long-horned beetle, Asian citrus psyllid, and stored product insect pests) that incorporate automated collection, processing, and analysis of insect acoustic signals and behavioral activity. Objective 5: Develop improved surveillance of invasive and outbreak insect pests (e.g., corn silk flies and kudzu bug) using visual-cue traps, and improve strategies for detection and prediction of such dispersing pests by understanding the role of visual and other stimuli affecting specific behaviors. Objective 6: Improve area-wide landscape management tactics by developing conservation biological control strategies to mitigate pest populations and attract or support natural enemies (e.g., against fall armyworm). Objective 7: Combine genetic methods with air-transport and climate modeling to describe and predict the distribution and behavior of agricultural pests to facilitate the mitigation of migratory source populations and to identify locations at high risk for infestations by invasive species such as fall armyworm, corn silk fly, soybean looper, Old World bollworm, and corn earworm.