Improvement of sample testing capacity for outbreak investigation of foodborne pathogens for FDA Vet-LIRN

FDA CVM Vet-LIRN PROGRAM GRANT PROPOSAL FOR FOA # PAR-18-604ABSTRACTThis grant proposal from the Ohio Department of Agriculture (ODA) is in response to FundingOpportunity Announcement (FOA) # PAR-18-604 from the Food and Drug Administration?s(FDA) Center for Veterinary Medicine (CVM) Office of Research Veterinary LaboratoryInvestigation and Response Network (Vet-LIRN).The purpose of this proposal from ODA?s Animal Disease Diagnostic Laboratory (ADDL) is toaddress the following key project areas:1. Increasing the capacity of laboratories conducting pathogen diagnostic testing by funding the updating or purchasing of additional equipment.2. Procurement of supplies needed for molecular testing efforts or methodimprovement.3. Develop/validate rapid tests to identify the source genera within animal food products.4. Purchase of equipment to improve the testing capacity, data analysis, and quality control of qPCR for Vet-LIRN foodborne pathogen caseinvestigations.5. Conduct increased testing in response to a new need identified by theVPO.This grant proposal addresses new technologies in molecular diagnostics that employ the use ofhigh-throughput, high-performance instrumentation and access to data via a secure Cloud. TheQuantStudio line of PCR systems are the latest advancements in qPCR instrumentation.Several additional documents including internal Proficiency Testing (PT) results (appendix ofResearch Plan), and certificate of AAVLD accreditation (appendix of Research Plan) areprovided along with the