1. Determine the incidence of Salmonella, E. coli and enterococci in swine fecal samples in the United States.<P> 2. Participate in the characterization of isolates at the phenotypic and genotypic level.
Approach: <P>
Fecal samples will be sampled using microbiological culture techniques to determine bacterial presence. Polymerase chain reaction (PCR) and Sensititre broth microdilution plates will be used to determine select antimicrobial attributes; pulsed field gel electrophoresis (PFGE) will be used to determine relatedness.