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<ol><li>Determine potential inoculation pathways by which pathogens/toxins are transferred to food crops when manure laden water is applied as irrigation.
<li>Determine potential inoculation pathways by which pathogens/toxins are transferred to food crops when covered with manure laden particulates.
<li>Determine any potential hazards when food crops are grown on land that has received manure.</ol>
Experimental Design: A pathogen/toxin profile will be developed for several types of waste water holding and treatment facilities and multiple sampling techniques will be used to determine pathogen/toxin profiles of dust leaving feedyards, manure storage areas, and soils having manure applied. Studies will be conducted at cooperating commercial feeding operations, in our research feedlot, in laboratory-scale systems, and during storage, treatment and application of manure to land. Once manure has been applied either through irrigation, dust, or normal land application; the life cycles of pathogens/toxins will be determined and followed. The effects of manure treatment and handling on populations of viable pathogens/toxins will be determined. <p>Previously was 6209-42000-001-00D (05/01).