INVESTIGATING CRYPTIC SPECIES DYNAMICS WITHIN PLASMOPARA VITICOLA

The major goals of this project include two (2) research objectives and three (3) training/career goals to improve genetic and infectivity knowledge involving the recently identified cryptic species of the grapevine pathogenPlasmopara viticola as well as refine the scientific, interpersonal and communication, and written skills of the Project Director. The project targets theAFRI Farm Bill Program Priority Area of "Plant Health and Production and Plant Products".Further characterization of the grapevine downy mildew crypticspecies (cl. riparia, aestivalis, and vinifera)will provide insight into infection and how it can be mitigated. Identification of effectors secreted from these organisms opensthe door to future analysis for use in breeding of resistance genes within commercial grape species that can reduce levels of infection in wine, juice, table, and raisin grapes worldwide--thus improving crop production.Research ObjectivesObjective 1:Perform an infection time-course comparison between P. viticola cryptic species using RNAseq analysis to determine differences in gene expression.Objective 2:Compare the effector repertoire of P. viticola clades vinifera and riparia to clade aestivalisTraining/Career goalsT&C Goal 1:Further develop molecular biology and computational skills.T&C Goal 2:Develop as a scientific leader and mentor.T&C Goal 3:Build upon collaborative efforts and science communication.