INVESTIGATIONS INTO SELENIUM TRANSPORT, ACCUMULATION, AND MITIGATION MECHANISMS IN WATERMELON FOR FOOD SAFETY USING GENOMIC TECHNOLOGIES

The primary aims of this research project are multifaceted, focusing on the genetic and environmental factors influencing selenium (Se) accumulation in watermelons, a concern due to potential health risks from soil contamination. Objective 1 aims to screen Genome-Wide Association Study (GWAS) panels to explore the natural variation in Se absorption, sequestration, and accumulation in watermelon, providing insights into the genetic basis of metal uptake and storage in plants. Objective 2 focuses on the functional validation of the genetic mechanisms and candidate genes identified in Objective 1, utilizing high- and low-accumulating watermelon cultivars. This approach will enable a deeper understanding of the genetic factors that govern Se accumulation and may lead to the development of cultivars with desired Se accumulation traits. Finally, Objective 3 seeks to evaluate high- and low-accumulating accessions in the field across various tissues, aiming to assess the environmental and developmental factors that influence Se distribution within the plant. This comprehensive approach combines genetic, molecular, and agronomic strategies to address the pressing issue of metal accumulation in watermelons, with the goal of enhancing food safety and understanding plant-environment interactions.