To Make Recommendations on the Best Practical Procedures to Sample and Test Poultry Flocks for Salmonella

This research project identifies optimal sampling and testing procedures for the detection of Salmonella spp. on the broiler farm.

<OL> <LI> Collate and update all information on sampling and testing practices from previous studies, industry, the literature and overseas.

<LI> Critically evaluate sampling and testing practices to determine the best procedures taking account of practicalities. The most promising sampling and testing procedures will be further evaluated using practical, laboratory-based studies to determine sensitivity and detection thresholds. A definitive sampling and testing plan will be validated in a number of broiler flocks by comparison with standard company monitoring and intensive sampling. On completion of technical evaluations a full cost/benefit analysis will be undertaken. This will take account of both the full costs of procedures and their technical merit.

<LI>Produce final recommendations in a format suitable for the production of guidance material for industry. A final report will be produced which will give economically viable recommended procedures to cover all stages of sampling and testing. The recommendations will include: a sample plan that will detect the maximum number of salmonella positive flocks; at what stage(s) of production should testing take place (i.e. age of the birds); the optimum method of sampling to assess flock status; how best to package and transport the samples to the laboratory; the laboratory test procedures(s) that will optimise detection of salmonellas of most concern to public health (particularly typhimurium and enteritidis); suitable rapid methods; alternatives to serotyping if available.