METHOD DEVELOPMENT AND VALIDATION OF OXFORD NANOPORE MINION FOR WHOLE GENOME SEQUENCING OF SAMONELLA SPECIES

Project summary ? Vet-LIRN Capacity 2018This Vet-LIRN capacity building project will enhance the ability of the lab to respond to animal or humanfood safety issues by acquiring training on, employing and evaluating a potential point-of-caretechnology to improve the speed and accuracy of disease diagnosis. This technology is the new OxfordNanopore Technologie?s MinION. The University of Minnesota Veterinary Diagnostic Laboratoryproposes 3 aims related to this technology, depending on the availability of funding.Aim one will involve up-to-date training on performing bacterial genome sequencing and genotypingprotocols on the MinION. In aim two we will then evaluate this MinION technology?s capability forwhole genome sequencing and genotyping of bacteria (specifically Salmonella) for disease detection andfor detection of antimicrobial resistance genes. In aim three, we will compare results using the MinIONwith our conventional whole genome sequencing and genotyping approach of Illumina MiSeqsequencing.