Methods for the detection of diverse parasites on packaged salads based on (viable) oocysts

This project will involve an approach for managing and monitoring produce safety to reduce the risk of foodborne illness from consumption of packaged salads: detecting human parasites on the surfaces of leafy greens in a rapid, accurate, and affordable manner. A novel test will simultaneously detect four key parasites that can be associated with produce-borne disease. Applying the newly validated tests in parallel with previously established methods for testing packaged salads will provide a realistic evaluation for the suitability of the developed assays for routine screening by the produce industry. Additional methods will determine the viability of these parasites and whether they are likely to cause disease to produce consumers if they are detected.