Identify mutations in L3 that confer resistance to DON and determine if FHB resistance can be engineered in transgenic wheat plants by expressing DON resistant L3 genes.
Approach: Characterize expression of the DON resistant L3 genes in transgenic wheat plants and determine if they will confer resistance to FHB. Analyze expression of PAP and L3delta in co-transformed wheat lines and determine if combination of these genes confers better resistance to FHB. Isolate DON resistant alleles of the wheat L3 genes for transformation into wheat.