MOLECULAR BIOLOGY, GENOMICS AND PROTEOMICS OF FOODBORNE PATHOGENS

1. Develop improved methods for rapid detection of human pathogens and protein toxins in food. 2.Develop microarrays and other multiplex methods to detect and analyze genera. 3.Delelop multi-locus sequence typing (MLST) of human pathogens for source tracking. 4. Develop methods for detoxifying protein toxins that may contaminate food. FY01 program increase $395.190. Add one SY 1. Development of gene and oligonucleotide microarrays and panarrays to identify foodborne pathogens, other threat agents and microbial and plant toxins. 2. Development of multiplex PCR assays for rapid detection of pathogens. 3. Validation of PCR- and microarray-based detection assays. 4. Detection, fingerprinting and virulence characteristics of microbial and plant protein toxins. FY05 program increase $ 695,000. Add one SY kmm:02/24/05 5. Development and validation of PCR- and microarray-based detection technoligies for the genera: Salmonella, Campylobacter, Listeria and Clostridium.
Program increase $44,550 FY06. 11/30/2005